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干货丨流速慢、回收率低、平行性差?SPE操作技巧帮你轻松解决
发布时间:2020-12-02   点击次数:64次

       WEISHENMESPECAOZUOZHONGHUICHUXIANLIUSUHENMANHUOLIUSUBUYIZHI、HUISHOULVDI、PINGXINGXINGCHADEQINGKUANGNI?ZHEIXIEWENTIKENENGHENDUOXIAOHUOBANDOUYUDAOGUO。SHIXIAOZHUXUANZEBUHESHI,FANGFAYOUWENTIMA?HAISHITIANLIAOLIJINGFENBUBUJUNYI,ZHUANGTIANSONGJINCHENGDUBUWENDING?QISHISPEXIAOZHUMEIGEPICIDELIUSUDOUYOUYANGEDEZHIKONGFANWEI,ZHIKONGFANWEINEIDEXIAOZHUNENGGOUBAOZHENGLIUSUDEWENDINGXING,ZHIKONGFANWEIZHIWAIDEXIAOZHUHUIBEIZUOWEIYICHANGPINCHULI。NEISHIBUSHICAIYONGWENDINGDEFANGFA,SHIYONGJINGCHUCHANGLIUSUJIANCEHEGEDEXIAOZHUJIUNENGWANQUANBIMIANZHEIXIEWENTINI?

       QISHIHAIYOUYIGEFEICHANGRONGYIBEIDAJIAHULVEDEXIJIEYEHUIDAOZHILIUSUGUOMAN:SPEXIAOZHUZAIYUNSHUGUOCHENGZHONGSHAIBANHETIANLIAOKENENGHUICHUXIANWEIXIAODESONGDONG,DAOZHIKONGQIJINRUTIANLIAO(YIBANBURONGYIBEIROUYANFAXIAN),HANYOUKONGQIDETIANLIAOZAIQIYADEZUOYONGXIADAOZHIRONGJILIUXIASUDUHENMAN。SHUODAOZHEILIDAJIAJIUNENGGOULIJIEWEISHENMETONGYIPIXIAOZHUHUICHUXIANLIUSUCANCHABUQIDEQINGKUANGLE。NEIWOMENZAISPESHIYANCAOZUOZHONGRUHEJIEJUEZHEIGEWENTINI?

 


一  排气泡

1.1 PAIQIPAODEFANGFA

√ 正压排除气泡

       YANGPINSHULIANGBIJIAOSHAO,TIANLIAOLIANGBIJIAOSHAO,RENGONGSHIJIAZHENGYABIJIAOFANGBIANDEQINGKUANGXIA,KEYISHIYONGZHUSHEQILIANJIEZHUANJIETOUDEFANGSHIJINXINGZHENGYAPAICHUQIPAO。

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<span class=

√ 负压排除气泡

       YANGPINSHULIANGBIJIAODA,TIANLIAOLIANGJIAODUO,RENGONGSHIJIAZHENGYABUFANGBIANDEQINGKUANGXIA,KEYISHIYONGZHENKONGBENGLIANJIEGUXIANGCUIQUZHUANGZHIDEFANGFA,FUYAPAICHUQIPAO。

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1.2 HESHIPAIQIPAO?

       SPECAOZUOZHONG,YIBANBAOHANHUOHUAPINGHENG、SHANGYANG、LINXI、XITUOSIGEBUZHOU。SHIMEIYIBUDOUXUYAOPAIQIPAOMA?DANGRANBUSHI,YIBANZAITIANLIAOZHIJIANYOUKONGXIDEQINGKUANGXIAXUYAOJINXINGPAIQIPAODECAOZUO,BIRUJIARUHUOHUAPINGHENGRONGYEZHIHOU,JIUXUYAOJINXINGPAIQIPAODECAOZUO,SHIDERONGYENENGGOUGENGHAODEYUTIANLIAOJIECHU。

       HAIYOUYIGERONGYIBEIHUSHIDEHUANJIEYESHIXUYAOPAIQIPAODE,YIBANLINXIHOUXUYAOJINXINGCHOUGANCAOZUO,CHOUGANHOUTIANLIAOJIANYEHUIYOUKONGQIJINRU,JIARUXITUOYEHOU,YESHIXUYAOJINXINGPAIQIPAOCAOZUODE,FOUZEDALIANGDEQIPAOYEHUIYANZHONGYINGXIANGXITUOYEDELIUSUYIJIXITUOXIAOGUO。

1.3 PAIQIPAODEZHUYISHIXIANG

       PAIQIPAOSHI,YIBANPAICHU1/3ZHUGUANTIJIDERONGJIJIKEPAICHUTIANLIAOZHONGDEQIPAO,QIEJIBUKEYIJIANGRONGYEPAIGAN。FANGZHIPAIGAN,SHOUDONGPAIQIPAODESUDUBUYIGUOKUAI,DANYEBUNENGGUOMAN,GUOHUANDESUDUKENENGHUIPAIBUCHUJIAODADEQIPAO。

       RUGUOHUOHUAPINGHENGRONGYEYIBUXIAOXINPAIGANLEZENMEBAN?ZHIYAOZHONGXINJIARUHUOHUARONGJIJINXINGPAIQIPAOJIUKEYILE。

       ZHENGQUEDEPAICHUQIPAOJIUKEYIZAIYIDINGCHENGDUSHANGBIMIANLIUSUGUOMANHUOLIUSUBUYIZHIDEQINGKUANGLE。NEIWOMENDEHUISHOULV、PINGXINGXINGJIUNENGDEDAOBAOZHENGMA?HAIYOUYIGESHENQIDEXIAOCAOZUO——CHOUGAN,TONGSHIZUOHAOZHEILIANGBU,CAINENGRANGWOMENDEDAOYIGEGENGHAODESHIYANJIEGUO。

 


二  抽干

 HESHICHOUGAN?

× 活化平衡溶液、上样液不需要抽干

       HUOHUAPINGHENGGUOCHENGDEZHUYAOMUDEYOULIANGGE,YISHIQINGXITIANLIAO,XIQUTIANLIAOZHONGBENDI,ERSHIBIANYUJIANHEXIANGFENZIDEZHANKAI。ZHUYAOSHIYIXIEZHANGLIANDEGUANNENGTUAN,LIRUC18ZAITIANLIAOSHIGANDEQINGKUANGXIABUSHIWANQUANZHANKAIDEZHUANGTAI,HUOHUAKEYISHITIANLIAOGUANNENGTUANSHIRUNHESHUZHAN,SHIZHIBAOCHIDUIMUBIAOHUAHEWUDEZUIDAXIFUHUOXING。

       SUOYIHUOHUAPINGHENGGUOCHENGSHIBUXUYAOJINXINGCHOUGANDE。

       YOUXIAOHUOBANCENGJINGWENDAOSHANGYANGYESHIFOUXUYAOCHOUGANCAOZUO?SHANGYANGYEYULINXIYEDEJIXINGYIBANSHIBIJIAOXIANGSIDE,SUOYISHANGYANGYEYESHIBUXUYAOJINXINGCHOUGANDE。

 

√ 淋洗液、洗脱液需要抽干

       LINXIYEXUYAOCHOUGANDEYUANYINZHUYAOYOULIANGGE:

       ①YIBANLINXIYEYUXITUOYEDEJIXINGXIANGCHAHUIJIAODA(RULINXIYESHISHUI,XITUORONGJIWEIZHENGJIWANHUOERLVJIAWAN),LIANGZHONGRONGJIBUHURONGHUORONGJIEXINGJIAOCHA,ZHEIHUIDAOZHIXITUOYELIUBUXIALAIHUOLIUSUJIAOMAN,ZUAIXITUORONGJIYUMUBIAOWUDEJIECHU,XITUOXIAOGUOCHA,HUISHOULVDI。

       ②LINXIYERUGUOSHISHUIDEHUA,BUJINXINGCHOUGAN,HUIYOUBUFENBEISHOUJIDAOXITUOYEZHONG,XITUOYERUGUOJINXINGDANCHUIDEHUA,HANSHUIDEXITUOYEYESHIHENNANJINXINGCHUIGANDE,RUGUOJINXINGZHANGSHIJIANDEDANCHUIJIANGSHAOLIANGDESHUIRONGYECHUIGANDEHUAHUIDAOZHIHUISHOULVPIANDIDEQINGKUANGCHUXIAN,SUOYILINXIZHIHOUSHIXUYAOJINXINGCHOUGANCAOZUODE。

       XITUOZHIHOUHUIYOUBUFENXITUORONGJICANLIUZAITIANLIAOSHANG,TIANLIAOLIANGJIAODUODESHIHOU,SHENZHIHUICANLIU1~2mLDEXITUOYE,WUFAWANQUANHUISHOUXITUOYE,DAOZHIHUISHOULVDI,PINGXINGXINGCHA。WEILEBAOZHENGXITUORONGJIYUMUBIAOWUWANQUANBEIHUISHOUYESHIXUYAOJINXINGCHOUGANDE。

总结

√ HUOHUAPINGHENG、XITUOQIANJINXINGPAIQIPAO。

√ LINXI、XITUOHOUJINXINGCHOUGAN。

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